This report documents the development of an interactive, web-based decision guide protocol for the selection, configuration, and location of wildlife crossings. For the first time, transportation planners and designers and wildlife ecologists have access to clearly written, structured guidelines to help reduce loss of property and life due to wildlife-vehicle collisions, while protecting wildlife and their habitat. The guidelines were based on goals and needs identified and prioritized by transportation professionals from across North America, and developed using the results of five parallel scientific studies.